Continuous mucociliary transport by primary human airway epithelial cells in vitro

Continuous mucociliary transport by primary human airway epithelial cells in vitro.

Mucociliary clearance (MCC) is an important innate defense mechanism that continuously removes inhaled pathogens and particulates from the airways. Normal MCC is essential for maintaining a healthy respiratory system, and impaired MCC is a feature of many airway diseases, including both genetic (cystic fibrosis, primary ciliary dyskinesia) and acquired (chronic obstructive pulmonary disease, br...

Transcriptional profiling of mucociliary differentiation in human airway epithelial cells.

When cultured at an air-liquid interface (ALI) in the appropriate medium, primary human airway epithelial cells form a polarized, pseudostratified epithelium composed of ciliated and mucus-secreting cells. This culture system provides a useful tool for the in vitro study of airway epithelial biology and differentiation. We have performed microarray analysis on ALI cultures of human bronchial ep...

Sildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro

Ion transport in epithelial spheroids derived from human airway cells

Pedersen, Peter Steen, Ole Frederiksen, Niels-Henrik Holstein-Rathlou, Per Leganger Larsen, and Klaus Qvortrup. Ion transport in epithelial spheroids derived from human airway cells. Am. J. Physiol. 276 (Lung Cell. Mol. Physiol. 20): L75–L80, 1999.—In the present study, we describe a novel three-dimensional airway epithelial explant preparation and demonstrate its use for ion transport studies ...

Ion transport in epithelial spheroids derived from human airway cells.

In the present study, we describe a novel three-dimensional airway epithelial explant preparation and demonstrate its use for ion transport studies by electrophysiological technique. Suspension cultures of sheets of epithelial cells released by protease treatment from cystic fibrosis (CF) and non-CF nasal polyps developed free-floating, monolayered epithelial spheres, with the apical, ciliated ...